| 质粒名称: | pAAV-CAG-tdTomato (codon diversified) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 59462 |
| 存储实验室: | Edward Boyden |
|
载体骨架基本信息 |
|
| 载体名称: | AAV with CAG promter |
| 载体出品公司: | Scott Sternson |
| 载体抗性: | 氨苄西林 |
| 空载体大小: | 4719 bp |
| 完整质粒大小: | 6150bp |
| 载体类型: | 哺乳动物表达，AAV |
| 宿生菌： | NEB Stable |
| 生长说明： | STBL3至30C（使用STBL3时使用卡宾西林）或DH5a至37C |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | tdTomato |
| 物种来源: | 合成的 |
| 插入大小: | 1431 bp |
| 基因突变: | 密码子多样化 |
| 启动子 | CAG |
| 克隆方法: | 限制性酶 |
| 5' 克隆位点: | 八木（未销毁） |
| 3' 克隆位点: | Ecori（未销毁） |
| 5' 测序引物 | gctctagagcctctgctaacc |
| 3' 测序引物 | gcagcgtatccacatagcg |
|
文献引用方法 |
|
| 材料和方法部分: |
pAAV-CAG-tdTomato (codon diversified) was a gift from Edward Boyden (Addgene plasmid # 59462 ; http://n2t.net/addgene:59462 ; RRID:Addgene_59462)
For viral preps, please replace (Addgene plasmid # 59462) in the above sentence with: (Addgene viral prep # 59462-AAV1), (Addgene viral prep # 59462-AAV2), (Addgene viral prep # 59462-AAV5), (Addgene viral prep # 59462-AAVrg), (Addgene viral prep # 59462-PHP.S), or (Addgene viral prep # 59462-PHPeB)
|
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pAAV-CAG-tdTomato (codon diversified) 6208 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION AAV-mediated expression of tdTomato (codon diversified) under the
CAG promoter.
ACCESSION .
VERSION .
KEYWORDS pAAV-CAG-tdTomato (codon diversified)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6208)
TITLE Accessory plasmids for optogenetics
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 6208)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6208
/organism="synthetic DNA construct"
/mol_type="other DNA"
repeat_region 2..131
/label=AAV2 ITR
promoter 442..719
/label=chicken beta-actin promoter
primer_bind 998..1020
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 1082..1101
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
regulatory 1140..1149
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
misc_feature 2601..3189
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2654..2674)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3072..3083)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3232..3353
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3243..3262)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 3297..3316
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
repeat_region 3515..3644
/label=AAV2 ITR
primer_bind complement(3762..3779)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3933..4521)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4013..4032)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4692..5552)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5315..5334
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5553..5657)
/gene="bla"
/label=AmpR promoter
rep_origin complement(5683..6138)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5820..5841)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 6032..6051
/label=F1ori-R
/note="F1 origin, reverse primer"
ORIGIN
1 gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt
61 tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac
121 taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt agccatgctc
181 taggaagagt accattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
241 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
301 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
361 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
421 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
481 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
541 tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg
601 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
661 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
721 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
781 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
841 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
901 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ctgtccgcgg
961 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
1021 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
1081 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgga tccggtaccg
1141 ccaccatggt gagtaagggc gaggaagtga tcaaagagtt catgcggttt aaggtgagaa
1201 tggaaggaag catgaacggc cacgagttcg aaattgaggg agaaggagag ggacggccct
1261 acgagggcac ccagacagcc aagctgaaag tgacaaaggg cgggcctctg ccattcgctt
1321 gggacatcct gagcccacag tttatgtacg gctccaaggc ctatgtgaaa catccagctg
1381 acattcccga ttataagaaa ctgagcttcc ccgaggggtt taagtgggaa agagtgatga
1441 acttcgagga cggaggcctg gtgactgtga cccaggacag ctccctgcag gatgggaccc
1501 tgatctacaa ggtgaaaatg agagggacaa attttccccc tgatggacct gtgatgcaga
1561 agaaaactat gggatgggag gcctccaccg aaaggctgta tccacgcgac ggggtgctga
1621 aaggagaaat ccaccaggct ctgaagctga aagatggggg acattacctg gtggagttca
1681 agacaatcta catggccaag aaacctgtgc agctgccagg ctactattac gtggacacaa
1741 aactggatat cacttcacac aacgaggact acactattgt ggagcagtat gaacggagcg
1801 aggggagaca ccatctgttc ctgggccatg ggactggaag taccggctca gggtctagtg
1861 gaaccgcctc aagcgaggat aacaatatgg ctgtgatcaa agagttcatg aggtttaagg
1921 tgcgcatgga gggcagcatg aatgggcacg aatttgagat tgaaggagag ggcgaaggga
1981 ggccttacga gggcacacag actgccaagc tgaaagtgac caagggagga ccactgcctt
2041 tcgcttggga tatcctgtct cctcagttta tgtacggaag taaggcctat gtcaagcatc
2101 ccgctgacat tcctgattac aagaaactgt ctttcccaga gggctttaag tgggagagag
2161 tgatgaattt tgaagatgga ggcctggtga ccgtgacaca ggactcctct ctgcaggatg
2221 gcactctgat ctacaaagtc aaaatgcgcg gcaccaattt tccacccgat gggcccgtga
2281 tgcagaagaa aacaatgggg tgggaggcca gcactgaacg gctgtatcct agagacggag
2341 tgctgaaggg cgaaatccac caggccctga agctgaaaga cggcggccac tacctggtgg
2401 agttcaaaac catctacatg gccaagaaac cagtgcagct gcccggctat tactatgtgg
2461 acaccaagct ggatatcaca tcccacaatg aagactacac cattgtggaa cagtatgaga
2521 ggtctgaagg acgccaccat ctgtttctgt acggcatgga tgagctgtat aagtaagaat
2581 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
2641 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
2701 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
2761 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
2821 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
2881 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
2941 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
3001 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
3061 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
3121 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
3181 cctccccgca tcgataccgt cgacccgggc ggccgcttcg agcagacatg ataagataca
3241 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
3301 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
3361 acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagca
3421 agtaaaacct ctacaaatgt ggtaaaatcg ataaggatct tcctagagca tggctacgta
3481 gataagtagc atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc
3541 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
3601 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcat taatgaatcg
3661 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc attaatgaat cggccaacgc
3721 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
3781 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
3841 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
3901 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
3961 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
4021 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
4081 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
4141 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
4201 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
4261 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
4321 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
4381 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
4441 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
4501 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
4561 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
4621 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
4681 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
4741 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
4801 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
4861 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
4921 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
4981 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
5041 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
5101 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
5161 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
5221 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
5281 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
5341 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
5401 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
5461 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
5521 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
5581 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
5641 caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata
5701 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
5761 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
5821 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
5881 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
5941 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
6001 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
6061 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
6121 cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat
6181 cggtgcgggc ctcttcgcta ttacgcca
//
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